碩士 / 中華醫事科技大學 / 生物醫學研究所 / 100 / Purpose of this study was to investigate chitosan in cosmetics in the antimicrobial activity, respectively, at different concentrations and different molecular weight and pH were measured inhibitory effect, filter out the best inhibitory concentration and pH, and the cell survival rate test, cytotoxicity test, heavy metal testing and microbiological testing and other tests.
Experimental use of sample to chitosan is mainly because of its better solubility than chitin. Chitosan non-toxic and biocompatible with Biodegradable, environmentally friendly polymer materials. In this study, we used three different molecular weight chitosan, the chitosan (MW:100,000~200,000 datons) were prepared as 0.5% (pH 3.2 and pH 5.6)、1% (pH3.3 and pH5.7) and 1.5% (pH5.7) chitosan (MW:100,000~300,000 datons) concentration of the preparation of 0.5% (pH 5.7), 1% (pH 5.7) and 2% (pH3.4) chitosan (MW:200,000~400,000 datons) concentration of the preparation into 1% (pH 5.7), and lactic acid as the solvent, the degradation of chitosan molecular weight, to improve the dissolution properties of chitosan the antibacterial agents antibacterial test analysis, experimental data and found that chitosan (MW:100,000~200,000 datons) at a concentration of 0.5% and the pH value of 5.6, that for Escherichia coli, Staphylococcus aureus all have antibacterial effects, the chitosan and chitosan (MW:200,000~400,000 datons) the inhibitory effect of poor; the same time, the concentration of 0.5%(pH5.6), 1%(pH5.7) chitosan (MW:100,000~200,000 datons) and a concentration of 1% (pH 5.7) of the chitin polymer sugar and lactic acid (control group) and a common antibacterial agent of cosmetics for cell survival and cell toxicity test, experimental results showed that lactic acid (control group) and common cosmetics and antibacterial agent with a cytotoxic cell-free survival, and different concentrations of chitosan on cell survival (MTT) and do not have good cytotoxicity (LDH).
The concentration of 1.5%, pH 5.7 chitosan (MW:100,000~200,000 datons) for heavy metals testing, test data results for the ND, namely, that chitosan does not contain heavy metals. In addition, the preparation of a concentration of 1%, pH 5.6 and 0.5%, pH 5.6 and 1.5%, pH 5.7 chitosan for microbiological testing, results showed that chitosan on Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and total plate count detection is ND, namely, that the study sample dose not contain the above strains (that is not detected).
| Identifer | oai:union.ndltd.org:TW/100HWAI7114005 |
| Date | January 2012 |
| Creators | Jhang, Ciouyun, 張秋筠 |
| Contributors | Cheng, Tsanhwang, 鄭粲煌 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 65 |
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