Heterotrophic Cultivation of Chlorella Protothecoides for Lipid Production / 異營培養小球藻(Chlorella protothecoides)於生產油脂之研究

碩士 / 明志科技大學 / 生化工程研究所 / 100 / Energy is an indispensable factor to develop our economic and living standard. As of today, fossil fuels such as coal, petroleum and natural gas have still served as the main global energy sources. But the growing consumption of fossil fuels has caused many environmental problems that threaten our ecosystem. Therefore, more and more countries actively to develop renewable energies as an replace energy to prevent the aggravation of global warming.
Biomass energy which stock in biomass is one of the important alternative energies in the world. Biomass energy can be divided into bioethanol, biodiesel, bioelectricity, hydrogen, and methane. Biodiesel is a promising candidate that can be used to substitute of petroleum diesel, because of a similar chemical structure and combustion efficiency with petroleum diesel. Furthermore, it is renewable, non-toxic fuel, and no net carbon dioxide or sulfur to the atmosphere and emits less gaseous pollutants compare with conventional diesel fuel.
In all kinds of biomasses, alga is one of the most potential species. Because use of microalgae to produce biodiesel would not compromise production of food, fodder and other products derived from crops. Furthermore, it can be systematically cultivated and harvested. After drying, lipid contained in algae can be extracted to produce bio-diesel. Therefore, microalgae have been studied as alternative feedstock for biodiesel production recently.
Because the green algae contain primarily C16 and C18 fatty acids with a high degree of unsaturation, and diesel fuel components (C13-18) have similar structures, recently there have been studies on using oleaginous algae as a source of biodiesel. In this study, sugarcane bagasse, the waste residue left after extraction of sugarcane juice, was used as a resource for fermentable sugar production. The waste bagasse was pretreated to remove lignin. The delignified bagasse was hydrolyzed using cellulase to produce fermentable sugar. The produced sugar was used as the carbon source for heterophic cultivation of Chlorella protothecoides. The efficiency of biomass,oil production,and fatty acid profiles from refined glucose and the alternative sugars were compared. The demonstrate that using the alternative sugar derived from sugarcane bagasse as the carbon source could obtain comparable biomass and lipid production. The highest biomass yield and lipid content were 13.2 g/L and 26.68 %, respectively.

Identiferoai:union.ndltd.org:TW/100MIT00723003
Date January 2012
CreatorsLin, Yu Sheng, 林育生
ContributorsSu, Chia Hung, 蘇家弘
Source SetsNational Digital Library of Theses and Dissertations in Taiwan
Languagezh-TW
Detected LanguageEnglish
Type學位論文 ; thesis
Format85

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