碩士 / 國立中興大學 / 生命科學系所 / 100 / The deep sea microbial community investigation can conjecture the marine associated environments, like oilfield, gas hydrate-buried site and hydrothermal vent, for further understanding the ecosystem and treasures of the natural resources. The CASQ box core MD178-3263C was obtained during the Marion Dufresne cruise (MD-178) at May 28, 2010. The sampling site of core MD178-3263C (8.52 meters) was located at the deep-basin area (21°32.490’N, 119°19.640’E) SW offshore Taiwan with water depth of 2819 meters. Both archaeal and bacterial 16S rRNA gene libraries were constructed from every 0.5 meter of core sections. Phylogenetic analysis indicated that the SAGMEG (34%) and MBG-D (33%) were dominant archaeal phylotypes, and the candidate division JS1 (36%) and Chloroflexi (22%) were dominant bacterial phylotypes. The occurrence of methanogenesis substrate- producing bacteria Firmicutes, Chloroflexi, Proteobacteria, Bacteroidetes and JS1, and methane producing archaea MBGD, GEG, SAGMEG and Methanomicrobiales, revealed the active methanogenesis process in this site. Thermophilic TMEG, SAGMEG and DHVC also present in this site. Additionally, the representative OTUs from most archaeal and bacterial phylotypes are close related with clones from deep sea sediments of active submarine mud volcano at East Mediterranean Sea, gas hydrate-bearing site and cold seep, which suggested that this habitat are potential gas hydrate-bearing site.
Identifer | oai:union.ndltd.org:TW/100NCHU5105023 |
Date | January 2012 |
Creators | Ding-Ju Chen, 陳丁濡 |
Contributors | 賴美津 |
Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
Language | zh-TW |
Detected Language | English |
Type | 學位論文 ; thesis |
Format | 67 |
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