碩士 / 國立中興大學 / 生命科學系所 / 100 / Recently, increasing of green-house gas CO2 concentration is the major factor for global warming. If we can use biological method for enhancing the fixation of CO2 gas, the global warming may be slowed down. In this study, we tried to transfer foreign gene into the bacteria to make their CO2 fixation more efficient and the candidate genes are ictB and sbtA from cyanonbacterium Synechocystis sp. PCC 6803. It’s has been reported that ictB, it can enhance photosynthetic activities and growth rate in transgenic Arabidopsis thaliana and Nicotiana tabacum plants. According to the previous study, we expect that the transfer of ictB and sbtA gene into bacteria can also enhance the growth rate for Spirulina platensis and Rhodopseudomonas palustris CGA009. For this reason, we constructed plasmid pUC-P-sbtA and pUC-P-ictB for S. platensis to improve the ability of carbon dioxide fixation. Besides, we also constructed another plasmid pMG-PpckA-ictB and pMG-PpckA-sbtA for R. palustris CGA009 to conduct the foundational physiology study and also expected the transgenic bacteria could grow faster. We found that transgenic R. s palustris grew faster than control in photoautotrophic condition. Then, C14-labeled bicarbonate was used to study the uptake rate of bicarbonate. Finally, we proposed a model about the metabolism of bicarbonate uptake in the transgenic bacteria.
| Identifer | oai:union.ndltd.org:TW/100NCHU5105029 |
| Date | January 2012 |
| Creators | Chun-Ying Wang, 王鈞瑩 |
| Contributors | Chieh-Chen Huang, 黃介辰 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 92 |
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