Studies of formalin application and residues analysis in Tilapia, Oreochromis hybrids / 福馬林在吳郭魚之應用及殘留檢測分析研究

碩士 / 國立中興大學 / 獸醫學系暨研究所 / 100 / Formalin (37% formaldehyde) is approved for use in aquaculture as a chemical for controlling ectoparasites and aquatic fungi in the US, Canada, and Asia while it is prohibited in Australia, Europe, and Japan because of its association with oncogenesis. Safety about formalin-treated aquatic animals used for food has been discussed in the aquaculture industry. The aim of this study is to determine the residual concentrations of formalin in muscle and liver in cultured tilapia (Oreochromis hybrids). Acute toxicities of formalin to tilapia were determined under a semi-static condition at 27℃. Talapia was bath treated with formalin at 25 and 50 ppm, respecilively. Our results showed that the median lethal concentration (LC50) lowered progressively as the duration of exposure increased. The toxicity curves became almost asymptotic with the time axis by 96h. The 48h-LC50 and 96h-LC50 was 179 and 177.2 ppm at 27℃, respeclivdy. After 50 ppm fotmalin treatment has been found to cause gill lamellar epithelia separation, hypertrophy, and necrosis under the histopathology examindion. Meanwhile, the time of undetectable residue amount of formalin in tilapia liver and muscle tissue at 25, 50 ppm was (0, 2) and (2, 2) hour, respectively. Formalin can be used at a concentration of 25 and 50 ppm in talapia without resulting in formaldehyde residues in the edible tissues.

Identiferoai:union.ndltd.org:TW/100NCHU5541027
Date January 2012
CreatorsChen-Hsuan Chang, 張鎮璿
ContributorsWay-Shyan Wang, 王渭賢
Source SetsNational Digital Library of Theses and Dissertations in Taiwan
Languagezh-TW
Detected LanguageEnglish
Type學位論文 ; thesis
Format76

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