碩士 / 國立成功大學 / 化學工程學系碩博士班 / 100 / Sodium alginate is a water-soluble natural biopolymer. Because of its nontoxicity, biodegradability and biocompatibility, it has been approved by FDA. In this study, we prepared a magnetite-sodium alginate/polyethylene oxide (Fe3O4-SA/PEO) nanofibrous mats. Fe3O4 were synthesized by chemical co-precipitation technique and loaded into sodium alginate-based nanofibers which have been electrospun by blending with a biocompatible, synthetic polymer PEO. To enhance hyperthermia effect, the Fe3O4-SA/PEO nanofibrous mats were placed around tumor followed by induction heating by high-frequency AC magnetic induction heater to generate the heat to kill cancer cells.
The morphology of composite and the distribution of Fe3O4 was observed by SEM and TEM micrographs. The results of XRD and SQUID showed the crystalline structure of Fe3O4 and superparamagnetism characteristic. Around 30wt% of Fe were presented in Fe3O4-SA/PEO nanofibrous mat as measured by ICP-MS. Besides, good heating efficiency in Fe3O4-SA/PEO nanofibrous mat was induced in magnetic field.
The cytotoxicity and in vitro hyperthermia effect for CT26 cells were evaluated by LDH assay and WST-1 test, respectively. In LDH test, on the first day, because of high concentration of Fe2+or Fe3+ was released from Fe3O4-SA/PEO, the cell death of this composite was approximate to 45% and it was higher than control group (medium only). However, there was no significant difference between Fe3O4-SA/PEO and control group on the third day. The hyperthermia effect of Fe3O4-SA/PEO was similar to pure Fe3O4 under the AC magnetic field. These results could attributed to the release of Fe2+or Fe3+ during the early cell incubation period. Therefore, it is essential to improve the methods for composite crosslinking as well as magnetite synthesis to decrease the release of iron ions and to ensure that the shape of Fe3O4-SA/PEO nanofibrous mat remained stable in the same time. We believe that Fe3O4-SA/PEO nanofibrous mats have excellent potential as an adjuvant hyperthermia for cancer treatment in the future.
| Identifer | oai:union.ndltd.org:TW/100NCKU5063118 |
| Date | January 2012 |
| Creators | Wan-JuChang, 張婉茹 |
| Contributors | Jui-Che Lin, 林睿哲 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 79 |
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