碩士 / 國立成功大學 / 生物科技研究所碩博士班 / 100 / In arthropods, the Down syndrome cell adhesion molecule (Dscam) is believed to be involved in “specific immune priming”. The extracellular region of arthropod Dscam can generate a great number of isoforms via mutually exclusive alternative splicing. This variability has been shown to function as a neural wiring adaptor in neural wiring and an antigen-specific receptor in arthropod specific immune responses. In shrimp Dscam, in addition to the extracellular region, cytoplasmic tail variants arise through the alternative splicing of 8 elements. These variants are potentially able to trigger different subsequent signal transductions. This study is the first to investigate the responses of shrimp Dscam during shrimp pathogenic infection by Vibrio harveyi and white spot syndrome virus (WSSV). Expression of shrimp Dscam and its variable cytoplasmic tail variants was stimulated by the injections of V. harveyi and WSSV. The Vibrio-induced shrimp Dscam isoforms were subsequently collected and subjected to a grouping analysis, which showed that half of the major Vibrio-induced shrimp Dscam isoforms were grouped into one clade that was clearly separated from the Dscam isoforms from the uninfected shrimp. An in vitro bacterial binding assay examined the interaction between different bacteria and rLvDscam isoforms. The data showed that the major Vibrio-induced Dscam isoforms have the binding affinity with V. harveyi. Based on these results, we believed that shrimp Dscam is involved in shrimp immunity, and the specific shrimp Dscam isoforms also have the ability to recognize V. harveyi.
| Identifer | oai:union.ndltd.org:TW/100NCKU5111112 |
| Date | January 2012 |
| Creators | Hsin-YiHung, 洪欣宜 |
| Contributors | Han-Ching Wang, 王涵青 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 87 |
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