碩士 / 國立成功大學 / 環境工程學系碩博士班 / 100 / Bioremediation is such a good way to treat petroleum pollutant since its low cost and environmental friendly acting. The objective in this study is to solve the bioavailability problems in soil and marine phases during bioremediation.
For contaminated soil, since the non-polar organic compounds would partition into soil organic matters (SOM). The objective in this part is to understand whether partitioning into SOM of petroleum hydrocarbons would affect the bioavailability or not. The experiment design is in batch module, using bioaugment technology to treat three kinds of target organic compounds, Heptadecane, Pristane and Naphthalene, in soil-water phase so as single- soil phase respectively, and the parameter is SOM ratio. The result shows that low SOM ratio condition did have better degrading efficiency in soil-water phase. However, in single-soil batch, different SOM ratio not only affects the primary biodegrading efficiency, but also applying different conditions such as pH, water content, and hydraulic conductivity of soil so that forming different microbe culture, that is, there are so many factors would affect the efficiency of biodegradation at the same time.
For oil spill out, the main factor of bioavailability is dilution. Thus, the objective in this part is to solve dilution problem by feeding back the immobilized microorganism which were enriched from native seawater at laboratory. Diesel is the target petroleum pollutant in this research. Take seawater samples around Taiwan to do the enrichment and the entrapment. After that, set serial batch tests to check the diesel removal efficiency and the long term activity of entrapped cells. The result shows that all the seawater samples did enrich oil degrading microbes successfully. The degrading efficiency was 47.25% for entrapped cells at virtual marine condition. Furthermore, if provide enough nutrients (phosphate and nitrate), the efficiency would up to 91.90%. That is, it’s necessary to provide extra nutrients while applying this methodology in the ocean. For the persistence test, the entrapped cells kept active for at least 356 days, even though passing long time starvation the entrapped cells still could do function.
| Identifer | oai:union.ndltd.org:TW/100NCKU5515039 |
| Date | January 2012 |
| Creators | Chih-HungChen, 陳緻紘 |
| Contributors | Liang-Ming Whang, 黃良銘 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 139 |
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