碩士 / 國立中央大學 / 化學工程與材料工程研究所 / 100 / Sodium alginate(ALG) is a biodegradable polyelectrolyte with negative charges, which may compete the positive polyelectrolyte with anionic DNA. Therefore, some reports indicated that alginate can interact with DNA/polycation and form a triple component complex. This particle is able to enhance the gene transfectrion efficiency due to electrostatic neutralization of the complex. Due to this property, we want to complex the three component with layer-by-layer(LbL) to enhance the transfection ability. So, in this study, we use different alginate concentration(0.004, 0.02, 0.1, 0.5 g/l) create the (DNA/PEI/ALG/PEI)4 LbL model and investigate the properties of DNA changes during the multilayer system measuring by ATR-FTIR, contact angle, quartz crystal microbalance (QCM), UV-vis, DLS and eletrophoresis gel. We observe there is two-fold DNA release at the 0.1g/l alginate assembly condition and the DNA adsorption increase with increasing alginate concentration. Also, the particle size which release from the multilayer increase too. At least, we use the multilayer to transfect the NIH/3T3 cells. Observing the transfction efficiency at 0.1g/l of (DNA/PEI/ALG/PEI)4 multilayer enhanced up to three times compare to (DNA/PEI)4 .
| Identifer | oai:union.ndltd.org:TW/100NCU05063094 |
| Date | January 2012 |
| Creators | Shiang-lung Tsou, 鄒翔龍 |
| Contributors | Wei-wen Hu, 胡威文 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 79 |
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