碩士 / 國立彰化師範大學 / 生物技術研究所 / 100 / English Abstract
Chromium (Cr) is released into the environment more than 100,000 tons per
year after industrial usage. It has been shown to be toxic and carcinogenic both
in vitro and in vivo, but little is known about its damage to aquatic embryos. In this study, we used zebrafish embryos as model to test Cr toxicity and its regulation on thioredoxin gene expression. The survival rate was 44% after 7.5mM Cr treatment for 12 h. Dose-dependent and time-course studies showed the LC50 was 7.5mM for 72h treatment. There are 16% teratogenesis were shown that included spinal column curving and edema in pericardial site. The reactive oxygen species were increased twofold and the lipid peroxidation was increased 2.5 fold after Cr treatment. Thioredoxin (Trx) is one of the important antioxidant enzymes in cell. The Trx gene expression was found major in notochord and head by whole-mount in situ hybridization. But the gene was inhibited up to 4 fold after Cr treatment for 6 h. We to observe survival rate was 46% that we knockdown Trx and There are 12% teratogenesis were shown that included spinal column curving and edema in pericardial site.The results showed Cr can inhibit Trx gene expression, and cause oxidative damage to fish embryos. We overexpression Trx to resist chromium (Cr) induced oxidative damage to zebrafish embryos
Key word: chromium, ROS, Trx
| Identifer | oai:union.ndltd.org:TW/100NCUE5108002 |
| Date | January 2011 |
| Creators | 林致和 |
| Contributors | 耿全福 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 100 |
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