碩士 / 國立高雄海洋科技大學 / 海洋生物技術研究所 / 100 / Shrimp white spot syndrome virus (WSSV) is one of the most devastating viral pathogens of cultured shrimp worldwide. Although adaptive immunity is believed to exist only in higher vertebrates, recent published papers showed the ability of WSSV structural protein VP28 to vaccinate shrimp and other crustaceans against WSSV. This study attempted to identify the joining proteins of the mentioned shrimp quasi-immune response at the translational level by proteomic researches. Two WSSV envelope proteins, VP28 and VP36B, were chosen as subunit vaccines expressed in Escherichia coli. Shrimp were intramuscularly injected with rVPs or PBS on day1 and day4 then on day7 gill tissues related as virus infected target were sampled. The two-dimensional gel electrophoresis (2-DE) patterns of the gill proteins between the vaccinated and PBS control shrimp were compared and 75 spots were selected for further analysis by the LC-ESI-MS/MS. The mRNA expression of the selected proteins was further assessed in gill and hemocyte using RT-PCR. The result identified the components of protein from rVP28 immunized shrimp whose expression were up regulated inferred joining the quasi-immune response such as cytosolic Manganese superoxide dismutase (cMnSOD), Calreticulin, Peroxiredoxin and phosphopyruvate hydratase. The information presented here should be useful to gain more insights into the mechanisms which are involved in the vaccine-inducing protection in penaeid shrimp.
| Identifer | oai:union.ndltd.org:TW/100NKIMT270005 |
| Date | January 2012 |
| Creators | Li-Hao Chen, 陳力豪 |
| Contributors | Jyh-Ming Tsai, 蔡志明 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 95 |
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