博士 / 國立屏東科技大學 / 熱帶農業暨國際合作系所 / 100 / The present study aimed to evaluate the effect of dietary supplementation of Saccharomyces cerevisiae P13 on: (1) the immune parameters including total haemocytes count, defferential haemocytes count, phenoloxidase acivity, respiratory burt, and superoxide dismutase activity, and (2) the resistance to Vibrio alginolyticus infection on white shrimp Litopenaeus vannamei. Shrimp were fed for a period of 4 weeks with diets containing S. cerevisiae P13 at 0 cfu kg-1 (control), and 107 cfu kg-1. Thereafter, white shrimp L. vannamei where divided into two groups and held in 20 ‰ seawater at 28 oC and 34 oC, respectively. The dietary supplementation of S. cerevisiae P13 at 107 cfu kg-1 evaluated at 28 ºC showed no effect on the total haemocyte count at any sampling times. However, THC of shrimps fed 107 cfu kg-1 supplemented diet transferred to 34 oC, showed significant different when compared with those fed the control diet at sampling times 12 and 24 hrs. All three types of haemocytes, granular cells (GC), semi-granular cells (SGC) and hyaline cells (HC), of shrimp kept at 28 oC and 34 oC, showed no significant difference between the shrimps fed 107 cfu kg-1 and those fed control diet. The phenoloxidase activity of shrimp kept at 28 oC showed no significant difference between the shrimps fed 107 cfu kg-1 and those fed control diet at any sampling time. In the other hand, the phenoloxidase activity of shrimps fed 107 cfu kg -1 supplemented diet transferred to 34 oC, showed significant difference at 24 hours. The respiratory bursts of shrimp kept at 28 oC showed significant difference between the shrimps fed 107 cfu kg-1 and those fed diet control at 48 hours, but no significant difference was observed at 0, 12, 24, and 96 hours. However, the respiratory bursts of shrimps fed control diet showed significant difference at 96 hours when transferred to 34 oC compared to those shrimps fed the 107 cfu kg-1. The superoxide dismutase activity in haemocytes of shrimp kept at 28 oC showed significant difference between the shrimps fed 107 cfu kg-1 and those fed control diet at 96 hrs, but no significant difference was observed at any other sampling time. The survival rate of shrimp, L. vannamei challenged with V. alginolyticus (2×106 cfu shrimp-1) over 24–144 hrs kept at 28 oC and 34 oC showed no significant difference between the shrimps fed 107 cfu kg-1 and those fed control diet. It is therefore suggested that L. vannamei fed with a diet containing S. cerevisiae P13 at leaves 107 cfu kg-1 for 4 weeks may have some potential to alter the immune ability and by this means, resulting in more enhancing resistance against V. alginolyticus.
| Identifer | oai:union.ndltd.org:TW/100NPUS5643003 |
| Date | January 2011 |
| Creators | Sixto Batista Santos, 席世多 |
| Contributors | Winton Cheng, Philip T. Cheng, 鄭文騰, 鄭達智 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | en_US |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 77 |
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