碩士 / 國立中山大學 / 海洋生物科技暨資源學系研究所 / 100 / Many bacterial diseases were controlled by antibiotics since mid 1900s.
However, over use of the drugs leads to the prevalence of resistant strains, some of
them are resistant to essentially all of the commercial antibiotics except for one or two.
Therefore, new drugs are needed to combat the die-hard resistant pathogens. This
study is aimed at isolating bacteria with antimicrobial activities from the intertidal
soils of Taiwan. A total of 25 samples were collected from 5 locations, including
Peng-Hu, Da-Peng Bay, Ken-ding, Gueishan Island and Little Liu-chiu. Marine Agar
2216 and Actinomycete Isolation Agar were used for cultivation. Of 313 bacterial
isolated, 47 of them showed antimicrobial activities against at least one of the 7
indicators (Bacillus cereus, Staphylococcus aureus, Klebsiella pneumonia, Salmonella
typhimurium, Pseudomonas aeruginosa, Vibrio harveyi, Escherichia coli). The 47
strains were classified based on 16S rRNA phylogeny. The majority of them are
Pseudoalteromonas spp. (31) and Bacillus spp. (9) as well as Virgibacillus sp. (1),
Vibrio sp. (1), Streptomyces sp. (1) and Microbulbifer spp. (4). Over all, 10/47 are
Gram positive and 37/47 require added salts for growth. The titers of antimicrobial
substances as judged by ethyl acetate extraction were influenced by cultivation
conditions, such as: growth temperature, types of media and time.
| Identifer | oai:union.ndltd.org:TW/100NSYS5270033 |
| Date | January 2012 |
| Creators | Ya-Ping Jhang, 張雅萍 |
| Contributors | Chi-Hsin Hsu, 徐基新 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 78 |
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