碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 100 / Pardaxin is a 33-amino acids antimicrobial peptide which first isolated from a mucosal secretion of the Red Sea Moses sole Pardachirus marmoratus in 1980. Besides the abilities of anti-bacterial and anti-viral, pardaxin also presents the antitumor activity. In this study, we hypothesize that pardaxin can inhibit murine fibrosarcoma cells growth. 13 μg/mL synthesized pardaxin caused 50% cytotoxicity in murine fibrosarcoma cell MN-11. We also revealed that pardaxin reduced anchorage-independent growth, cell migration ability, and caspase 3/7 activity in MN-11 cells. Moreover, intratumoral injection of pardaxin could attenuate tumor growth in vivo by inducing apoptosis, inhibiting inflammatory response, suppressing pro-inflammatory cytokines secretion, and diminishing angiogenesis.
Due to the side effects of chemotherapy and increase in
chemotherapy-resistant cancers, it is urged to develop therapies that provide specific toxicity in cancer cells and reduce damages to normal tissues without inducing drug resistance. This thesis exhibits that pardaxin has an antitumor activity with expect on its efficacy in clinical application in the future.
| Identifer | oai:union.ndltd.org:TW/100NTOU5086027 |
| Date | January 2012 |
| Creators | Shu-Ping Wu, 吳淑萍 |
| Contributors | Cheng-Hui Lin, Jyh-Yih Chen, 林正輝, 陳志毅 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 90 |
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