碩士 / 國立臺灣海洋大學 / 食品科學系 / 100 / Ornamental fish are vulnerable to stress during transportation. The aim of this study was to create a natural anti-stress drop for guppy to increase stress tolerance in transport. The main objectives are the following: (1) to design a simulated transport model to evaluate the effect of GTE (Glycine tomentella) extract drop in packing water of guppy (Poecilia reticulate); (2) to standardize the application method of using GTE drop during logistics; (3) to identify the functional compounds and the anti-stress effects of GTE; (4) to determine the storage stability and shelf- life of the GTE drop for improving the survival rate of guppy. Cumulative survival rate during simulated transportation of guppy packed in GTE drop containing 6.25-37.5 μg/mL showed 0% survival while the control group was 19%. GTE concentration was reduced to 25-100 μg/L, resulted in increased survival rate (88-98%) being higher than that of the control (60%) and the ethanol group (60%), based on equal volume of active agent added. Survival rate was highest (98%) for the GTE 100 μg/L group. GTE 100-125 μg/L groups showed survival rate of 89-90% higher than the control (82%) and the commercial drop (Aquaguard) group (74%). The 3 groups including control, ethanol and Aquaguard showed deterioration in water quality, fungal infection on the dead fish and hyperplasia of the chloride cells, fusion of secondary lamellae and hyperemia of the gill tissue in the survived fish after simulated transport. GTE drop added into packing water improved these phenomena. The pro-inflammatory responses (TNF-α and PGE2) were significantly increased in gill tissue of guppy exposed to stressors. GTE significantly inhibited TNF-α (not detected) and PGE2 expression (4133 ± 714.1 pg/mg protein), being lower than the control (3.54 ± 2.15 and 7667.9 ± 1405.2 pg/mg protein respectively) and the Aquaguard group (0.90 ± 0.68 and 6245.9 ± 546.0 pg/mg protein respectively). Expression of TNF-α and PGE2 in gill tissue can be used as marker of inflammatory responses of small fish of which blood sample was not easy to draw. GTE drop was also effective to improve the stress tolerance of other ornamental fishes including Sciaenochromis ahli, blood parrot fish (Cichlasoma citrinellum × C. synsilum) and Metriaclima greshakei better than the Aquaguard. However, the GTE drop at the concentration used did not show positive effect in the gene modified fluorescent zebra fish TK-2. The efficacy and optimal dosage of GTE drop varies with fish species. Guppy has provided an ornamental fish model to prove that GTE can be applied as a stress-tolerance enhancing agent for the ornamental fish logistics. The anti-stress bioactive compounds of GTE were identified with HPLC. Total polyphenols, total flavonoids, tartaric acid, p-hydroxybenzoic acid were present in the GTE extract. The GTE drop stored in light-proof plastic bottles for 12 months at room temperature or 40℃ showed no significant (p＞0.05) differences in color, pH and functional compounds from the initial preparation.
| Identifer | oai:union.ndltd.org:TW/100NTOU5253024 |
| Date | January 2012 |
| Creators | Tzu-Yun Huang, 黃子芸 |
| Contributors | Bonnie Sun Pan, 孫寶年 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 111 |
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