碩士 / 國立臺灣海洋大學 / 食品科學系 / 100 / Hypogonadism and oxidative stress occurs commonly in men with type 2 diabetes associated male infertility. The aim of this study was to investigate the capability of anti-inflammatory and anti-oxidative on fucoxanthin, and to evaluate the protective effects on male reproduction of fucoxanthin against diabetic rats. In vitro, concentration 6.25 g/mL of 97% fucoxanthin significantly inhibited NO production, ,reactive oxidative spices production in lipopolysaccharide (LPS) induced RAW 264.7 macrophage cells. In animal experiment, the model diabetic rat was induced by nicotinamide (230 mg/kg) and streptozotocin (65 mg/kg). 40% fucoxanthin was tested in three doses (13, 26 and 65 mg/kg, p.o daily) for 2 and 4 week. Besides, rosiglitazone (RSG) administration (0.571 mg/kg) as positive control. The results indicate that any dose of fucoxanthin administration for 2 weeks improve hyperglycemia and insulin resistance, sperm numbers, motivity and lipid peroxidation. But only 26 and 65 mg/kg of fucoxanthin could significantly restore KiSS1, GPR54 mRNA expression, activity of anti-oxidative enzyme, scavenging free radical. Any dose of fucoxanthin administration for 4 weeks improve insulin resistance and leptin resistance, and restored sperm motivity, decreased abnormal sperm number, inhibit lipid peroxidative. Besides,it has restored GPR54 and SOCS-3 mRNA expression in the hypothalamus, and recover LH and testosterone level. In conclusion, not only the antioxidant and antiinflammatory property but also steroidogenesis effect of fucoxanthin might have contributed for its ability to decrease the type-2 diabetes mediated insulin and leptin resistance, and protect male reproduction.
| Identifer | oai:union.ndltd.org:TW/100NTOU5253044 |
| Date | January 2012 |
| Creators | Yu-Chun Hsu, 許育珺 |
| Contributors | Zwe-Ling Kong, 龔瑞林 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 138 |
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