碩士 / 國立臺灣海洋大學 / 食品科學系 / 100 / Sorghum distillery residue (SDR), a by-product from spirit fementation, contains 14% crude protein. Therefore, it is worthy to evaluate the potential of utilizing SDR as an ingredient in commercial shrimp feed to reduce the cost of protein source used in feed production and solve the waste disposal problem caused by the SDR. To develop an in vitro digestibility test of SDR simulating the in vivo digestion in white shrimp, activities of the major digestive proteases including total proteinase, trypsin, chymotrypsin and carboxypeptidase A & B were studied.
The proteinase activity from midgut gland of white shrimp (Litopenaeus vannamei) was found maximum at 60℃ with two optimal pHs of 2.0 and 7.0. The maximal trypsin and chymotrypsin activities were detected at pH 8.0. Carboxypeptidase A and B showed the highest activities at pH 3.0.
In vitro test was performed using enzymatic extracts from midgut gland of white shrimp to digest in two steps, first at pH 8.0, 60℃ for 30 min followed by digestion at pH 3.0, 60℃ for 30 min. For ex vivo digestibility, midgut glands of shrimp cultured for 8 weeks in in vivo test were taken out to do in vitro digestibility as described above.
There was no significant (P > 0.05) difference in in vitro digestibility between N-100 soybean meal (49.22% crude protein) and fish meal (47.33% crude protein), but significantly (P < 0.05) higher than SDR with or without polyethylene glycol. SDR treated with 0.5% PEG resulted in higher protein digestibility (27.03%) compared to SDR without PEG (12.31%).
In vitro and ex vivo digestibility of two kinds of iso-protein feeds, Feed A containing 14% N-100 soybean meal and 6% porcine and fish meal, and Feed B containing 14% porcine and fish meal and 6% N-100 soybean meal, showed that soybean meal can replace animal protein up to 14% in feed formulation for white shrimp. There is a difference in in vitro and ex vivo digestibilty of feeds added with SDR and without SDR. In the in vitro test, no significant (P > 0.05) difference in protein digestibility between feeds added with 20% SDR and without SDR for white shrimp. However, shrimp fed diets without SDR had higher specific growth rate and protein efficiency ratio than shrimp fed diets containing 20% SDR in the ex vivo test.
| Identifer | oai:union.ndltd.org:TW/100NTOU5253055 |
| Date | January 2012 |
| Creators | Nguyen Thi Thu Trang, 阮氏秋妝 |
| Contributors | Bonnie Sun Pan, 孫寶年 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | en_US |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 57 |
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