碩士 / 國立臺灣海洋大學 / 食品科學系 / 100 / There are many reasons for skin damage like burns, cuts, abrasions and ulcers. Wounds were vulnerable infected by microorganisms if it is exposed to air for a long time. In the recent, the studies focus on accelerating wound repair. It is known that basic fibroblast growth factor (bFGF) promoted fibroblasts migration, proliferation, and keratinocytes growth and effective helped tissue repair and skin wound healing. However, the use of bFGF is limited by two drawbacks, which are it’s short half-life and easily degradated by enzyme. In this study, we fabricate fucoidan-modified chitosan/alginate scaffold to protect and control release bFGF and evaluate effect on wound healing.
The experimental results show that the volume ratio of chitosan to alginate at 3:1 was porious, swellable and degraded slowly. After sulfation of fucoidan, the –OH group on C-4 was replaced by –SO3 and the sulfated degree increased to 43% which is called F43. 1% F43 had effective anti-oxidation properties, good biocompatibility and protected bFGF from trypsin degradation. 1% F43-modified chitosan/alginate scaffold (1% F43-S) are suitable biomaterials because of suitable degradation rate, good biocompatibility, and anti-inflammation properties. Moreover, bFGF loaded 1% F43-S could maintain bFGF activity and control its release. The concentration of bFGF reached 2.37 ng/mL for 72 hours release. In vitro studies show that bFGF loaded 1% F43-S promoted fibroblasts proliferation, migration, and keratinocytes growth. In brief, bFGF loaded 1% F43-S effectively helped the wound repair and will have a great potential in clinical applications in the future.
| Identifer | oai:union.ndltd.org:TW/100NTOU5253097 |
| Date | January 2012 |
| Creators | Huang-Yu Zeng, 曾皇諭 |
| Contributors | Yi-Cheng Huang, 黃意真 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 100 |
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