碩士 / 國立臺灣海洋大學 / 海洋生物研究所 / 100 / Abstract
Total of 10,961 fish eggs were towed by using 1000 μm Round Mouth Ichthyoplankton net (RMI) from bottom to surface at 25 stations in the East China Sea in July, 2009 (cruise OR1_905, 8,933 eggs), and at 6 stations in April (cruise OR1_924, 603 eggs) and July (cruise OR1_932, 1,425 eggs), 2010 respectively. All fish eggs were classified to 105 morpho-types by morphological characters. Among them, only 10 types could be identified to 7 order, 10 types to family, 1 types to genus and 9 types to species level, including 11 taxa (belonging to 7 orders, 9 families, 7 genera and 6 species), and the other 75 types couldn’t be identified, and the percentage of successful identification was 16.36 %. But with DNA barcoding, 7 types were identified to family, 6 types to genus, 64 types to species and 4 types to over 2 taxa in the same types (belonging to 10 order, 33 families, 45 genus and 40 species), and the percentage of successful identification was 83.52 %. Besides, the results of morpho-types of Bothidae and Trichiurus lepturus by morphological method were found to be Samaridae and Trichiurus japonica, respectively. Therefore, fish eggs identification by using DNA barcoding was more accurate and could solve the group that couldn’t be identified by morphological method in the past. However, remaining 24 types failed be identified (4 types were not available in the database, and 20 types failed to extract DNA).
The dominant species of fish eggs in summer, 2009 were Auxis rochei, Cynoglossus arel and Cynoglossus interruptus etc. There were some seasonal and annual differences among the dominant species at the 6 stations in the spring and summer cruises. The major dominant species in spring were Chelidonichthys kumu, Chauliodus sp. and Dentex tumifron etc. In summer, 2009 cruise were Auxis rochei, Minous monodactylus and Champsodon sp. etc. and in summer, 2010 cruise were Cynoglossus interruptus, Auxis rochei and Harpadon nehereus.
All stations in summer, 2009, could be divided into 5 groups (A- E). The major contribution species of A group (inshore group) were Coryphaena hippurus and Cynoglossus interruptus, B group (mid-shore and offshore group) were Auxis rochei, Diodon holocanthus and Trichiurus japonicas, C group (s22) was Trichiurus japonicas, D group (sK) was Cubiceps sp. and E group (upper Yangtze river estuary group) was Erisphex pottii. The distributional pattern of fish eggs was different from adult fish or larvae, but it was fitted well with the pattern of surface temperature, chlorophyll a and salinity. In this study, there were 13 economic species in the top 20 dominant species in the spring and summer cruises. Thus, the East China Sea is an important spawning ground of many economic species. This information should be important for planning and establishing the Marine Protected Area (MPA) or seasonal closures.
Keywords: East China Sea, Barcoding of Life, Fish eggs, Species composition
| Identifer | oai:union.ndltd.org:TW/100NTOU5270003 |
| Date | January 2012 |
| Creators | Han-Yang Lin, 林翰揚 |
| Contributors | Kwang-Tsao Shao, I-Shiung Chen, 邵廣昭, 陳義雄 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 230 |
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