碩士 / 國立臺灣海洋大學 / 機械與機電工程學系 / 100 / This paper presents chitosan film formation by the microelectrode of adjusting and controlling immediately, on applying to microarray cells and cells measurement, that can co-culture many kinds of cells are detected together and drug screening. By using electrolysis of water in Chitin aqueous solution to produce a localized pH change in the aqueous solution, To make chitosan deposited on the electrode surface with Alkaline region in aqueous solution.
According to the characteristic of hydrogenated amorphous silicon (a-Si:H), which impedance diminished after light illuminating. Not only used light to create real electrodes, but also combined optics setup with DMD projector to be the microelectrode of adjusting and controling immediately. Electrode patterns can be changed quickly and efficiently, and this method replaces the traditional microelectrode especially. Therefore it can control the positions and forms of calcium alginate gel formation what we want.
This research defines resolution of chitosan film, the diameter of light spot or aluminum electrode are D0, and the diameter of chitosan film is D, hence D/D0 define as resolution of chitosan film,and to explore the affect of chitosan solution concentration, deposition time, current density and aluminum electrode.We got a resolution of 1.02 by deposition of chitosan films at a current density of 4A/m2 for deposition 3 minutes, but the minimum size of the chitin membrane without aluminum electrode chips is 800um.If deposition of chitosan films on aluminum electrode chips at a current density of 4A/m2 for 3 minutes,we can improve the minimum size of chitosan to 50um ,and we got a resolution of 1.01. Aluminum electrode chip can improve the resolution and minimum size of the chitin membranes.
| Identifer | oai:union.ndltd.org:TW/100NTOU5491017 |
| Date | January 2012 |
| Creators | Lu-Shiuan Wei, 韋祿軒 |
| Contributors | Shih-Hao Huang, 黃士豪 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 64 |
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