碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 100 / In order to determine the concentration of different pigments from phytoplankton, we developed a method by adding trans-β-Apo-8’-carotenal as the internal standard to either the DHI pigment standards or phytoplankton extraction samples followed by the liquid chromatography separation with uv detection. Since each phytoplankton has its own specific pattern of various pigments, we used CHEMTAX to estimate the contribution from each phytoplankton to different collection spots. The quantitative analysis of phytoplankton pigment samples were collected from spring (April, 2009) and summer (July, 2009 and July, 2010) in East China Sea. We tried to build up a model to explain the phytoplankton community change during Yangtze River flood as well as in spring and summer seasons in the East China Sea.
We used 31 psu of salinity as the indicator for fresh water flood impact from Yangtze River. The salinity value less than 31demonstrates the positive effect from fresh water flood. The surface water samples from twelve of forty-seven stations show their salinity measurement less than 31psu in OR1-905. However, surface water samples from twenty of thirty-seven stations have salinity less than 31 psu in OR1-932. Therefore, the fresh water flood had dramatically impact to the East China Sea in July, 2010 (OR1-932). On the other hand, only six of thirty-two stations show salinity of surface water sample less than 31 psu in spring season (OR1-897). The fresh water came from the coast and we named it as Yangtze River coastal water.
From the analysis results of all surface water samples, chlorophyll a provided from bacillariophyceae had the major contribution in OR1-932 rather than ORI-905; however, the cyanophyta have reversed effect in contribution of chlorophyll a than bacillariophyceae. This finding indicates that bacillariophyceae was the dominant phytoplankton in the environment with high nutrient salt, especially during the severe flood of Yangtze River. The cyanophyta, on the other hand, was the major phytoplankton I the low nutrient salt environment. Further, from the OR1-905 and OR1-907 results, we found that prymnesiophyceae and bacillariophyceae were the major species in the spring season especially when the water temperature was low.
According to the results from OR1-897, OR1-905, and OR1-932, we can conclude that bacillariophyceae is the dominant species in the deep water and preferred in the condition with high nutrient salt especially during the server flood of Yangtze River. Cyanophyta, on the other hand, prefer the environment with low nutrient salt. Dinoflagellate grows along the salinity of 31 psu but easily be affected by other dominant phytoplankton. Therefore, we need more data to evaluate if Dinoflagellate could be as an indicating phytoplankton for environment change in the future.
| Identifer | oai:union.ndltd.org:TW/100NTOU5613023 |
| Date | January 2012 |
| Creators | Yin-Hua Su, 蘇胤華 |
| Contributors | Pang-Hung Hsu, Tung-Ming Hsiung, 許邦弘, 熊同銘 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 126 |
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