碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 100 / Basic-helix-loop-helix (bHLH) transcription factor plays an important role in the process of vertebrate embryonic development, and the hypoxia -inducible factors (HIFs) play a key role to adapt physiological functions during hypoxic stress. NeuroD is a basic helix loop helix transcription factor that is widely expressed in CNS and neuronal cells. NeuroD regulates cell cycle and control neuronal differentiation. Knock down hif2α diminished survivin (birc5a and birc5b) expression and caused severe neuronal apoptosis and impaired neuronal differentiation. In addition, blocking hif2α translation also repressed neurod transcription. However, it is unclear whether other neurod members are also influenced by hif2α suppression. Here I found that the neurod and neurod4 are the major neurod members expressed in retina and CNS during development. A less amount of neurod2 transcript is also observed in retina and CNS regions during development. neurod6a and neurod6b are weakly expressed in specific CNS regions during development. Knockdown hif2α expression suppressed all members of neurod expression, except neurod6b, Knockdown birc5a and birc5b expression also decreased all members of neurod mildly. In summary, this study revealed that neurod, neurod4 and neurod2 are expressed in retina and CNS during development and their expressions were influenced by hif2α, birc5a and birc5b depletion.
| Identifer | oai:union.ndltd.org:TW/100NTOU5613035 |
| Date | January 2012 |
| Creators | Wei-Yi Chen, 陳瑋毅 |
| Contributors | Chin-Hua Hu, Shiow-Yi Chen, 胡清華, 陳秀儀 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 44 |
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