碩士 / 國立臺灣大學 / 醫學工程學研究所 / 100 / Liver diseases have threatened people’s lives in the world. Especially in Taiwan, liver diseases are the major cause of death throughout the years. Alcohol, drugs, virus-induced hepatitis, and malignant tumor may cause liver damage and result in liver failure. In the treatment of liver failure, liver transplantation has been established as the most effective option. However, there is limitation due to the shortage of donated organ supply, and the immunosuppressive therapy must be administrated to prevent immunological rejection of transplanted tissue. In this decade, hepatic tissue engineering, which includes hepatocyte transplantation, tissue engineered grafts and liver assist devices (LADs), has been investigated in attempt to provide alternative approaches. One of the major limitation of hepatic tissue engineering is the short-term viability and rapid de-differentiation of hepatocytes in vitro. To overcome this problem, recent studies have indicated the combination of appropriate scaffold, growth factor, and co-culture with other types of cells when culturing hepatic cells.
In this study, porous sponge made by silk fibroin is used to be the 3D scaffold in hepatic tissue engineering. Porous scaffold is like the 3D structure of extra-cellular matrix (ECM) in morphology and provides suitable microenvironment for higher seeding density and exchange of nutrients, oxygen, and metabolites. Silk fibroin is the biodegradable and biocompatible material, and it can provide good mechanical properties. Silk fibroin conjugated with lactose can approve the formation of spherical cell pellet to mimic 3D microstructure of hepatocyte in vivo.
By observing the interaction of hepatocytes and lactose-cyanuric chloride-silk fibroin porous sponge manufactured by lyophilization, the results demonstrated that hepatocytes had good viability and normal metabolic function while culturing over a long period of time. On the other hand, hepatocytes co-cultured with the nonparenchtmal cells may approve the hepatocellular specific functions. Although the cell-cell interaction and the soluble factors between hepatocytes and nonparenchtmal cells requires further understanding, the combination of lactose-cyanuric chloride-silk fibroin porous sponge, hepocytes, and nonparechymal cells co-culturing system has the potential in hepatic tissue engineering application.
| Identifer | oai:union.ndltd.org:TW/100NTU05530033 |
| Date | January 2012 |
| Creators | Yun Hung, 洪昀 |
| Contributors | Yi-You Huang, 黃義侑 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 47 |
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