博士 / 國立臺灣科技大學 / 材料科學與工程系 / 100 / In this study, novel anti-adhesion mat made of chitosan (ChS) and alginate (AL) were fabricated via electrospinning. The electrospinning parameters were varied to investigate the effect of each parameter on the performance of the mats. The viscosity, electrical conductivity, surface tension, and specific gravity of the spinning dope were measured. These fibers exhibited a sheath-core structure that was revealed using a confocal laser scanning microscope (CLSM) and fluorescence-labeled polymers. The morphology of electrospun mat was examined using a field-emission scanning electron microscope (FE-SEM) for the fiber size. The average diameter of the fibers ranged from 600 to 900 nm depending on the electrospinning parameters.
To mimic the stability of alginate fibers in physiological fluids, the release of alginate from these fibers in normal saline and DMEM solution was also tested. The results demonstrated that the sheath-core structure of alginate fiber can greatly reduce the degradation by 40% for 3 days in physiological environment. In addition, the degree of disintegration in DMEM for chitosan alginate (AL-ChS) mat was 64% of that of conventional calcium alginate (AL-CaCl2) mat after 5 days. The swelling ratio, complete blood count (CBC), and reactive oxygen species (ROS) were evaluated against the anti-adhesion barrier. The results of CBC and ROS tests showed that no adverse effect was observed for these anti-adhesion barriers. Tissue anti-adhesion potential was evaluated with in vitro cell adhesion model and in vivo rat model. About 40% of the animals treated with chitosan alginate exhibited no tissue adhesion between injured peritoneum and cecum. Both macroscopic and histological observations showed that chitosan alginate mat was highly effective in reducing the formation of postoperative abdominal adhesions in vivo. Therefore, electrospun chitosan alginate mat could be a tissue anti-adhesion barrier owing to its efficient tissue anti-adhesion performance.
| Identifer | oai:union.ndltd.org:TW/100NTUS5159018 |
| Date | January 2012 |
| Creators | Jung-Jhih Chang, 張榮志 |
| Contributors | Ming-Chien Yang, 楊銘乾 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 184 |
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