碩士 / 國立臺灣科技大學 / 材料科學與工程系 / 100 / In this study, fibers with a core-shell structure were prepared via electrospinning alginate (AL) into chitosan (CS) solution. Inorganic hydroxyapatite (HAp) particles were added into the resulting fibrous mat. In addition, a third layer of collagen was coated onto the AL-CS composite fibrous mat to form a three-tier structure.
The viscosity of the electrospinning solution was measured. The morphology of the electrospun mat was examined using scanning electron microscopy (SEM), transmission electron microscopy (TEM) and laser confocal microscopy (CLSM). The stability of the structure of the fibrous mat was assessed in various media. The cytocompatibility was evaluated with osteoblasts and fibroblasts based on cytotoxicity and cell proliferation.
Results from SEM and TEM showed that HAp particles were successfully embedded in the AL-CS-Col composite fibrous mat. Stability assessment showed that the release of collagen from the mat was significantly reduced. Cytocompatibility tests showed that the mat exhibited no cytotoxicity, and cells can proliferate on culturing with the mat.
These results indicate that the AL-CS-Col exhibited positive potential as scaffold for bone tissue engineering.
| Identifer | oai:union.ndltd.org:TW/100NTUS5159028 |
| Date | January 2012 |
| Creators | Yu-cheng Lin, 林育成 |
| Contributors | Ming-chien Yang, 楊銘乾 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 91 |
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