碩士 / 國立臺灣科技大學 / 化學工程系 / 100 / Cyanophycin (CGP) is a non-ribosomal synthesis protein which is composed aspartic acid, arginine and lysine. The lysine molecular, which contains primary amine, can allow chemical modifications with anhydride grafting. In this study, we use different types of anhydride and attempt to expand the applications of the cyanophycin.
For characterization of structure, we used FTIR to analyze the functional groups of cyanophycin modified with anhydride and TGA to detect the thermal stability.
For DNA/polymer complex tests, the encapsulation ability follows the order of CGP-octanoic anhydride > CGP-acetic anhydride > CGP-myristic anhydride with DNA electrophoresis. Especially CGP-octanoic anhydride can completely encapsulate DNA, so we can use this result to explore the transfection efficiency of animal cells.
For biocompatibility, the cytotoxicity tests of cyanophycin modified with anhydride uses MTT method after cells incubate for 48 hours. We can find that cyanophycin modified with anhydride comparing to controlling improve apparently, especially in low concentration. In addition, the effect of the protein test in the immune response is also the same result for Raw 264.7.
To sum up, the cyanophycin modified with anhydride provides a better condition for cell growth. If the product of cyanophycin modified with anhydride can further modification, can be drug carriers. Also we hope to have the opportunity to look forward to the application in biomaterials area.
| Identifer | oai:union.ndltd.org:TW/100NTUS5342048 |
| Date | January 2012 |
| Creators | I-Ting Tsai, 蔡依廷 |
| Contributors | Wen-Chi Tseng, 曾文祺 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 80 |
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