碩士 / 國立臺灣科技大學 / 化學工程系 / 100 / Cyanophycin granule polypeptide (CGP) is a non-ribosomal protein found in cyanobacteria. CGP which is composed of aspartic acid (back bone), arginine and lysine (side chain) is produced with Escherichia coli through recombinant technology. Gelatin whose sources are animal skin ,bone and connective tissue is large and complex polypeptide of different amino acids, then arranged in different ways to form a triple helix.
Both of CGP and gelatin are well biocompatible. In recent decades, gelatin is widely used in biomaterials. However, CGP exhibits poor mechanical properties which limit its possible applications. In this study, to improve the applicability of CGP, we made CGP crosslink with gelatin by glutaraldehyde reaction.
In the beginning, we air-dried the mixed CGP/gelatin solution on teflon plate. After that we made the films crosslink with glutaraldehyde. The films were removed after crosslinking, and then we used universal test machine to test their mechanical properties. The result exhibits both tensile strength and elongation decreased with higher mixing ratio of CGP. In the swelling studies, the CGP/gelatin films were immersed in phosphate buffered saline at 37 ℃. We obtained the result that the swelling trend was downward with higher mixing ratio of CGP. Finally, using scanning electron microscopy to observe the surface morphology of the films, we found it smooth and homogeneous. They didn’t show cracks or holes when we removed the film froms teflon plate or during the crosslinking.
| Identifer | oai:union.ndltd.org:TW/100NTUS5342082 |
| Date | January 2012 |
| Creators | BAU-LUO WU, 吳保羅 |
| Contributors | none, 曾文祺 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 60 |
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