碩士 / 國立臺灣科技大學 / 化學工程系 / 100 / Cyanophycin (CGP) is a natural polymer. The structure of recombinant CGP consists of a poly-aspartic acid backbone with arginine and lysine as side chains. Due to the biocompatibility of cyanophycin, it can be used in biomaterial applications.
Hydroxyapatite (HAP) is an important biomaterial used in hard bone tissue substitute because of its bioactive and excellent osteoconductivity. In this study, cyanophycin/ hydroxyapatite composites film was prepared by coprecipitation method to enhance the osteoconductivity of cyanophycin.
The physical and chemical property of cyanophycin/hydroxyapatite films were analyzed by scanning electron microscopy, Fourier transform- ed infrared spectroscopy, X-ray diffraction and contact angle. The cell viability was testd by using MTT assay and the cell morphology was observed with SEM. The alkaline phosphatase (ALP) activity was measured by using enzymatic colorimetry.
The experimential results showed that the hydroxyapatite mixed with hydrophobic significantly higher. The cyanophycin/hydroxyapatite films exhibited rounghness when the surface morphology was observed by SEM. The cell viability showed minimal toxicity when Saos-2 cells on the CGP/HAP flims for 48 hr；the Saos-2 cells on CGP/HAP film also had improved ALP activity .
In conclusion, CGP/HAP film can guide bone cell differentiation. It has a great potential of biomaterials applications for bone tissue engineering.
| Identifer | oai:union.ndltd.org:TW/100NTUS5342088 |
| Date | January 2012 |
| Creators | Yen-chih Chen, 陳彥志 |
| Contributors | Wen-Chi Tseng, 曾文祺 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 62 |
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