碩士 / 靜宜大學 / 食品營養學系 / 100 / Sterculia lychnophora is one of the popular Chinese herb medicine which is used to relieve sore throat and cough traditionally. In this study, we extracted the polysaccharides from Sterculia lychnophora and investagated the chemical compositions, antioxidant activity and anti-inflammatory of the crude polysaccharides. The Ames test was used to evaluate the toxicity and mutagenic potential of the crude polysaccharides. Three extracted methods including water (SLW), 50% methanol (SLM) and 50% ethanol (SLE) extractions were used to extract polysaccharides from the seeds of Sterculia lychnophora. The results indicated that the yield of crude polysaccharides were similar among the three extracts, which were 19.95%, 21.54% and 19.09% for SLW, SLM and SLE respectively. The monosaccharide compositopns of crude among three crude polysaccharides were the same, including rhamnose, arabinose, galactose and glucose. The antioxidant activity of crude polysaccherides increased with the increase of concentration of SLW, SLM and SLE. The EC50 of DPPH radical scavenging, ABTS radical scavenging, and Ferrous metal ion chelating activity of crude polysaccharides ranged in 0.71-1.35 mg/mL, 1.88-46.13 g/mL, and 2.22-3.61 mg/mL, respectively. In vitro of anti-inflammatory of crude polysaccharides showed the added dose of 500 g/mL could promote the growth of RAW264.7 cells. As the dose of crude polysaccharides decreased to 62.5 g/mL, the best inhibitory of NO production were 50.75%, 56.17% and 39.51% for SLW, SLM and SLE, respectively. In the assessement of food safety, the Ames test was used to analyze the mutagenic potential of crude polysaccharides of Sterculia lychnophora. The results indicated the negative tests in toxicity and in mutagenic potential from S. tyhimurium TA were obtained according to the biological assay of Ames test.
| Identifer | oai:union.ndltd.org:TW/100PU000255006 |
| Date | January 2012 |
| Creators | Fan, Liling, 范俐伶 |
| Contributors | Wang, Chiunchuang, 王俊權 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 115 |
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