碩士 / 靜宜大學 / 食品營養學系 / 100 / Leukemia is the twelfth of cancer causes of death in adult and the first cancer of death in children in Taiwan. Recently, there is an increasing interest in the study how to prevent or delay the leukemia development. Caulerpa microphysa (grape algae) belongs to the Chlorophyta division. In this study, we examined the antioxidant activities of Caulerpa microphysa pepsin-digested extract (CME) and its possible apoptosis mechanim in leukemia cells (WEHI-3 and HL-60).
The results demonstrated that the CME had 2,2’-azinobis ( 3-ethylbenethiazoline-6-sulfomic acid ) (ABTS+) radical-scavenging activity and Fe2+-chelating ability. In the cells model, the CME can decrease the cell viability of leukemia cells. CME induced chromatin condensation and DNA damage by DAPI staining and comet assay, respectively in WEHI-3 and HL-60 cells. Furthermore, CME induced DNA fragments by DNA gel electrophoresis in WEHI-3 cells. Flow cytometry assay demonstrated that CME induced cell cycle arrest in the G0/G1 phase and stimulated the reactive oxygen species (ROS) production and calcium release, but loss of mitochondrial membrane potential (MMP). Our results indicated that the protein levels of cyclin D, cyclin E, CDK6, CDK2 and Bcl-2 were decreased, and that of p21, p27, p53, Bax, Bid, GRP78, GADD153, AIF, caspase-3 and caspase-9 were increased which were examined by western blotting in WEHI-3 cells after CME treatment. In addition, CME decreased the glutathione levels, however, increased malondialdehyde levels, glutathione peroxidase and catalase activities in WEHI-3 cells.
In conclusion, CME had possess antioxidant activity and induced growth inhibition in WEHI-3 and HL-60 cells. CME induced G0/G1 phase arrest, apoptotic morphological change and DNA fragmentation, decreased MMP, increased the Ca2+ release and ROS production and changed oxidant-antioxidant system in WEHI-3 cells. Our results suggest that CME may have the anticancer potential and antioxidant activity.
| Identifer | oai:union.ndltd.org:TW/100PU000255011 |
| Date | January 2012 |
| Creators | Chuang, Meiyu, 莊梅鈺 |
| Contributors | Chou,Sutze, 周淑姿 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 108 |
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