碩士 / 東吳大學 / 微生物學系 / 100 / Waste water generated in the daily life will into the sewage treatment plant for processing, and brominated organic pollutants contained in wastewater, such as dibromo-diphenyl ether, decabromodiphenyl ether, tetrabromobisphenol A, etc., will accumulate in the sludge of sewage treatment plants the sludge resources to produce risk. The previous reserch shows the strain has the ability to degrade or enzyme can remove a variety of organic pollutants. But add to the degradation ability strains, due to environmental conditions may lead to added strain can not be effective to achieve the removal of organic pollutants. In this study, strain laboratory by Erhjen river isolated PBDE degradation ability of strain, Bacillus sp. (W1) and Pseudomonas sp. (W4), and lignin enzymes through solid culture extraction of the enzymes were extracted in the matrix of the enzyme and the cultivation of White rot fungi. In this study, alginate micro-embedding technique to observe the strain has the ability to decompose and extracellular lignin degradative enzymes containing bromine organic pollutant removal ability after embedding and embedded.
In the batch experiments, we found that the dibromo ether after processing seven days to remove the order of the W1 strain (100%)> W4, strains (92%)> lignin degradative enzymes (83%). DecaBDE removal experimental results, the strain of the added W1, W4 strains and enzymes DecaBDE can not reach the effect of biodegradation. Tetrabromobisphenol A removal capacity of the display order for enzymes of lignin (90%)> W1, strains (22%)> W4, strains (19%).
In the degradation of tetrabromobisphenol A and dibromo-diphenyl ether experimental results show that by embedding the enzyme capsule removal efficiency and embedding and micro-embedding of the removal efficiency was no significant difference. Speculated that the reasons may be after the alginate entrapped porosity greater than the lignin extracellular enzymes size, so the enzymes are free to move inside and outside the capsule, with or without the use of embedded technology does not affect the enzyme on the pollutant removal efficiency.
In the column system experiment results show that the continuous pollutants from entering the column in response to contaminants adsorbed on the column of the sludge after its removal reaction added to the strain or enzyme. Finally, out of the water of pollutants are reduced to very low concentrations or not detected. Column system in practical application to remove the bromine-containing contaminants with good removal efficiency.
| Identifer | oai:union.ndltd.org:TW/100SCU05381008 |
| Date | January 2012 |
| Creators | Shao-wen Hsu, 徐紹文 |
| Contributors | Bea-ven Chang, 張碧芬 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 93 |
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