碩士 / 大仁科技大學 / 製藥科技研究所 / 100 / In this study, the ripe fruits, roots, different fermentation periods of fruits (3, 4, 6, 9 and 12months) named 3MF, 4MF, 6MF, 9MF and 12MF and different fermentation periods compound of fruits (3 and 20months) named 3MCF, 20MCF of Morinda citrifolia L. produced by the Pingtung County were selected to be the research materials. The DDPH free radical, xanthine oxidase (X.O) inhibition and hydrogen peroxide scavenging activities were performed to survey their antioxidant capacity.
The different fermentation periods of fruits were directly filtrated, mature fruits and roots were dissolved in distilled water, 50% and 95% ethanol, then boiled for 4 hours at 60-70℃. All of the filtrate was concentrated under vacuum, the concentrates were then freeze-dried 48 hours by drying method to get the dry powders of fruit water(FW)、water alcohol50% (FWA50%), alcohol95% (FA95%) and root water(RW)、water alcohol50% (RWA50%), alcohol95% (RA95%) extracts which were dissolve in DMSO to prepare the stock solution and executed their antioxidant activities.
The 3MFand FWA50% revealed significant DPPH radical inhibition activites and the inhibition percentages were 54±9% and 66±8% at sample concentration (1000μg/ml). The 20MCFand FWA50% showed significant xanthine oxidase (X.O) inhibition activites and the inhibition percentages were 58±0% and 54±0%. The RA95%、FWA50% and 6MF displayed significant hydrogen peroxide scavenging activities and the scavenging percentages were 100±8%,100±0% and 99±0%. Our result suggested the ripe fruits, roots and different fermentation periods of fruits of Morinda citrifolia L. possessing antioxidant activities.
The homocysteine injuried coronary arteries were also performed to survey their cardiovascular protection effect. The vasodilator percentages of the control and Hcy injuried group were 46±6%, 33±8%, and the ones of 3MF, 20MCF and FWA50% at sample concentration (250μg/ml) were 61±23%, 58±13%, and 59±13%. Our result suggested that the ripe fruits, roots and different fermentation periods of fruits of Morinda citrifolia L. were able to protect the coronary arteries against Hcy damage.
| Identifer | oai:union.ndltd.org:TW/100TAJ05549017 |
| Date | January 2012 |
| Creators | Shin Chin, 伍欣晶 |
| Contributors | none, 郭代璜 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 59 |
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