碩士 / 慈濟大學 / 分子生物暨人類遺傳學系碩士班 / 100 / MicroRNAs (miRNAs) are small non-coding RNAs that play as post-transcriptional regulators in various cellular processes. MicroRNA-132 (miR-132) has been shown to affect dendritic growth and spinogenesis in cultured hippocampal neurons and in brain slices. It has been known that p250 GTPase-active protein (p250GAP) and methyl-CpG-binding protein 2 (MeCP2) translation is regulated by miR-132. However, its physiological effects in vivo and correlation with behavioral tasks remain unclear. Therefore, this thesis aims to investigate the role of miR-132 in the hippocampal region of mice after fear conditioning. Half an hour after trace fear conditioning, miR-132 expression level was increased in the bilateral hippocampi. Next, engineered miR-132 antagomer carried by lentivirus to was infused to the hippocampi to knockdown local miR-132 expression and the effect was correlated with fear learning behavior. MicroRNA-132 expression level was successfully knocked down by 20 %, which was associated with significant learning deficit in trace fear conditioning. Furthermore, level of p250GAP and cAMP response element binding protein (CREB) was not significantly different when miR-132 knockdown in fear learning behavior. Additionally, level of miR-132 of trace fear conditioning (TFC) group was significantly higher than Naïve group in the left hippocampus after retrieval of contextual fear memory and asymmetrical expression pattern of miR-132 was observed. Moreover, after retrieval of contextual fear memory knockdown of miR-132 the in hippocampus increased expression of MeCP2 and p250GAP, but did not change CREB. Knockdown of miR-132 did not change freezing behavior to context, suggesting that miR-132 expression may affect later processes related to memory consolidation instead of retrieval of contextual memory per se.
| Identifer | oai:union.ndltd.org:TW/100TCU05498007 |
| Date | January 2012 |
| Creators | Ruo-Yu Wang, 王若羽 |
| Contributors | Ingrid Y Liu, 劉怡均 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 66 |
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