博士 / 國立臺北科技大學 / 工程科技研究所 / 100 / Piscidin-1, a 22-residue cationic peptide isolated from mast cells of hybrid striped bass, has potent antimicrobial activities against both gram-positive and -negative bacteria. To date, there is no report of its antitumor activity on any tumor cell lines. In this study, we examined the antitumor activity of a synthetic piscidin-1 peptide against several human cancer cell lines by an MTS assay and soft-agar colony formation assay. Results indicated that a low dosage of piscidin could induce both apoptosis and necrosis in HT1080 cells according to Annexin-V/propidium iodide and acridine orange/ethidium bromide staining, and triggered a necrotic cell death pathway in a short period of high-dosage treatment. The destruction of cell membranes by piscidin-1 was demonstrated by transmission electron microscopy. Furthermore, piscidin-1 could also inhibit the migration of HT1080 cells in a dose-dependent manner. To sum up, this study provides the first evidence of the anticancer activity of the antimicrobial peptide, piscidin-1, and illustrates a promising way to treat cancer.
Transgenic fish cannot seek permission to market is due to the reason that field test research area and basic research technology development for evaluation biosafety are not available. Infertility is a necessary adjunct to the exploitation of transgenic fish unless in completely secure land locked facilities. In addition, infertility is an important parameter in its own right, even aside from its use for containment of GM fish. In this research project, we applied Cre-loxP technology to zebrafish for infertility technology development. We produced fish carrying the plasmid that contained apoptosis genes, and these apoptosis genes are separated by two loxP sites under an ovary-specific promoter, AMH, or ZPC promoter control. After obtaining the transgenic lines with both transgenic fish crossed with each other, the floxed DNA would specifically be eliminated from the female, and apoptosis gene expression would be turned on in ovary cells. The infertility technology can be applied to transgene fish industry.
| Identifer | oai:union.ndltd.org:TW/100TIT05028032 |
| Date | January 2012 |
| Creators | Heng-Ju Lin, 林恆如 |
| Contributors | Yeh-Fang Duann, 段葉芳 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 78 |
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