碩士 / 國立雲林科技大學 / 環境與安全衛生工程系碩士班 / 100 / The study was mainly concerned with the toxic effects of the DBP on zebrafish three developmental stages (embryo, larvae and adult fish). The results show that zebrafish embryo under different exposure time of 24, 48, 72 and 96 hours result in the different LC50(median lethal concentration) value at 2.5 mg/L, 1.1 mg/L 0.8 mg/L and 0.5 mg/L respectively. Adult zebrafish LC50-96hours is 8.68 mg/L. Larvae LC50-12hours is 3.33 mg/L. Showing zebrafish DBP tolerance is adult> larvae> embryo. DBP can serious effects to reproductive capacity. Zebrafish can spawn after self-adjustment, but the quantity less than normal. In histological examination, sample-gill can found tissue swelling, tissue injuries and cell fusion. Liver tissue of zebrafish as exposed for 5 days, inflammation and necrosis are the most widely recognized forms of hepatocyte cell death. After exposed for 10 days, we can found apoptosis and cell exfoliation in the liver. After one to two days of hatching, low-dose response (<0.7mg/L) in Zebrafish Embryos have yolk-sac enlargement. In the DBP exposure, it is clear that the zebrafish had the growth anomalies.
| Identifer | oai:union.ndltd.org:TW/100YUNT5633048 |
| Date | January 2012 |
| Creators | Chia-che Shen, 沈佳哲 |
| Contributors | none, 袁又罡 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 67 |
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