碩士 / 中國醫藥大學 / 針灸研究所碩士班 / 101 / Acupuncture had been widely used to treat disease, such as epilepsy, stroke etc. However, effect of acupuncture on neuroprotection and enhancing neuronal regeneration remain unclear. Therfore, the purpose of the present study was to investigate the effect of acupuncture on neuronal cell damage, we injected KA (kainic acid ) 1μg/μl to rat right hippocampal CA1’s edge, and then observed these rats’ behaviors while epilepsy attacked. We selected these rats which fitted above the 4th stage of Racine’s classifications and 150-200 times of wet-dog shakes after KA injection. Afterwards, screened rats were randomly assigned to PBS group, KA group, sham group and EA (electro-acupuncture) group (2Hz; GB8 and GB9 acupoints on the right side), there were six rats in each group. We sacrificed rats and removed brain tissue out of their heads and made brain sections 7th day after KA administration. We used HE stain, and NeuN and BDNF immunohistochemistry stain respectively to observe rats’ hippocampal tissue and neurons. The results showed that KA could destroy hippocampal tissue, but sham and elector-acupuncture reduced these damages. In the CA1 region of the hippocampus, the count of NeuN stain positive cells was 188±9.32 in PBS group greater than 17±3.71 in KA group, 59±3.20 in sham group, and 128±5.42 in EA group (all p < 0.05); the count of NeuN stain positive cells in EA group was greater than that in KA group and sham group (both p < 0.05); the count of NeuN stain positive cells in sham group was greater than that in KA group ( p < 0.05). In the CA3 region of the hippocampus, the count of NeuN stain positive cells was 137±5.50 in PBS group greater than 6±2.44 in KA group, 30±2.75 in sham group, and 50±2.86 in EA group (all p < 0.05); the count of NeuN stain positive cells in EA group was greater than that in KA group and sham group (both p < 0.05); the count of NeuN stain positive cells in sham group was greater than that in KA group ( p < 0.05). In the CA1 region of the hippocampus, the count of BDNF stain positive cells was 301±5.50 in EA group greater than 26±3.87 in PBS group, 6±1.77 in KA group, and 150±5.85 in sham group (all p < 0.05); the count of BDNF stain positive cells in sham group was greater than that in KA group and PBS group (both p < 0.05); the count of BDNF stain positive cells in PBS group was greater than that in KA group ( p < 0.05). In the CA3 region of the hippocampus, the count of BDNF stain positive cells was 53±3.11 in EA group greater than 8±1.97 in PBS group, 3±1.34 in KA group, and 32±3.08 in sham group (all p < 0.05); the count of BDNF stain positive cells in sham group was greater than that in KA group and PBS group (both p < 0.05); the count of BDNF stain positive cells in PBS group was greater than that in KA group ( p < 0.05).
Based on the results of mention-above, we infer that 2Hz EA on GB8 and GB9 acupoints have neuroprotection on kainic acid-induced hippocampal neuronal damage.
| Identifer | oai:union.ndltd.org:TW/101CMCH5108009 |
| Date | January 2013 |
| Creators | Cheng-Hao Huang, 黃正豪 |
| Contributors | 沈淵瑤 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 40 |
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