碩士 / 嘉南藥理科技大學 / 生物科技系 / 101 / WSSV is a new large double-stranded DNA virus that infects aquatic crustaceans, and causes damages and losses to cultured shrimp. The around 300-kb genome of WSSV Taiwan isolate ( WSSV-TW ) encodes 532 putative open reading frames ( ORFs ). Some of them have been characterized encoding structural proteins of WSSV virions and many nonstructural proteins transiently expression during virus infection. It is study, time-course study using by real-time PCR showed that the wssv071 ORF of WSSV-TW was expressed after 24 hours of infection. The result suggested that wssv071 might be a late gene. Furthermore, Yeast-two hybrid analysis showed that WSSV071 protein might interact with the N-terminal and C-terminal peptide fragments of shrimp transglutaminase ( TGase ).
| Identifer | oai:union.ndltd.org:TW/101CNUP0111014 |
| Date | January 2013 |
| Creators | Po-Yuan Chang, 張博淵 |
| Contributors | Nai-Yueh Tien, 田乃月 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 76 |
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