碩士 / 中山醫學大學 / 醫學檢驗暨生物技術學系碩士班 / 101 / There are four isozymes of alkaline phosphatase (ALP) in human, tissue nonspecific alkaline phosphatase (TNAP), placental alkaline phosphatase (PLAP), germ cell alkaline phosphatase (GCAP), and intestinal alkaline phosphatase (IAP). Recent studies showed that TNAP was not only involved in the mineralization, but also involved in calcification. Previous study showed that TNAP expressed at early development stages of zebrafish, especially at kidney, the adult organ for hematopoiesis that was similar to the bone marrow of mammals. In zebrafish, erythrocytes, macrophages and neutrophils generated the major regulation of kidney. The aims of study were to elucidate the effect of TNAP on zebrafish macrophage development and function. After TNAP morpholino injection, the zebrafish embryogenesis was delayed, the width and length of eyes and yolk extension were smaller and shorter than those of wild-type control, respectively. Using MPO stain, the number of neutrophils decreased in the caudal hematopoietic tissue (CHT). By neutral red staining, the number of macrophages increased in the retina and posterior trunk. In this study it induce macrophages function of migration, phagocytic and lysis decrease. In conclusion, ALP plays very important roles in macrophage development and function.
| Identifer | oai:union.ndltd.org:TW/101CSMU5108001 |
| Date | January 2013 |
| Creators | Tsung-Han Lee, 李宗翰 |
| Contributors | Jine-Yuan Hsieh, 謝錦源 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 53 |
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