碩士 / 中臺科技大學 / 生物科技研究所 / 101 / Sewage discharge had become the main source of water pollution in Taiwan. Usually, physical and chemical methods were used to monitor the disposal of sewage in early stage. Physical and chemical methods enable to measure the categories and concentrations of pollutants in the sewage, but unable to know whether the pollutants will cause toxic reactions in aquatic organisms. That’s why the biomonitoring of environmental pollutions were always used with the physical and chemical methods. In the study, we used Zebrafish (1.5±0.1 cm) to monitor the campus of experimental and life water. The experimental project of 96 hrs acute toxicity was to observe the lethal rate, histopathologic sections of gill, acetylcholinesterase (AChE) activity and metallothionein (MT) concentrations. The experimental project of 30 days chronic toxicity was to observe the lethal rate and condition factor (CF). In 96hr acute toxicity test, the lethal rate was 100% in experimental and life wastewater but 10% lower than sewage disposal treatments. In Gill histological sections, experimental wastewater showing secondary lamella degeneration and RBC cluster, life wastewater showing swollen bases of secondary lamella, sewage disposal treatments were similar to control. AChE activity of the sewage disposal treatment(Lo: 78.63 U/mg; Eo: 60.77 U/mg) was higher than the experimental (31.73U/mg) and life wastewater(47.51U/mg). MT concentrations of the sewage disposal treatment (Lo: 339.73ng/m; Eo:375.76 ng/m) was lower than the experimental (623.29 ng/mg) and life wastewater (599.11 ng/mg). In chronic toxicity test, the lethal rate of the sewage disposal treatment (Lo: 45.55%; Eo: 43.33%) was lower than the experimental (60.00%) and life wastewater (53.33%). Condition factors of control (1.39) was higher than the waste water treatment group (Li: 1.24; Lo: 1.32; Ei: 1.27; Eo: 1.33). In summary, the waste water treatment systems of Central Taiwan University of Science and Technology could effectively decrease most acute toxicity of wastewater. But the remained trace waste still inhibited the growth rate, oocyte yield and hatching rate in chronic toxicity.
| Identifer | oai:union.ndltd.org:TW/101CTC07105001 |
| Date | January 2013 |
| Creators | Wang, Guan-Jen, 王冠鎮 |
| Contributors | Yuan, Yu-Chen, Tsai, Shu-Chuan, 袁又宸, 蔡淑娟 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 101 |
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