碩士 / 大葉大學 / 環境工程學系碩士班 / 101 / In this study effects of culture conditions on growth of Nannochloropsis sp. were examined using response surface methodology and a mathematical modal was established to find the optimal condition for biomass production. The appropriate range of each factor was determined for growth with the method of one-factor-at-a-time. Then central composite design was set up for factors of sodium bicarbonate concentration,salt concentration and pH based on the results obtained.
The results by one-factor-at-a-time method showed that the suitable culture condition for Nannochloropsis sp. was with 20 g/L of sodium bicarbonate and pH 8.5, 0.3 g/L of sodium nitrate in medium. Based on the resuts by one-factor-at-a-time, a central composite design of three factors, each with two levels, was designed, and the experments were carried our and results were analyzed with statistical software, Minitab. The optimal culture condition for Nannochloropsis sp. was as follows: sodium bicarbonate 30.8 g/L, solt 20.3 g/L, and pH 8.27, and biomass 1 g/L.
| Identifer | oai:union.ndltd.org:TW/101DYU00515019 |
| Date | January 2013 |
| Creators | Jhin-heng Chuen, 闕志衡 |
| Contributors | Shih-Chung Yu, 余世宗 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 85 |
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