碩士 / 高苑科技大學 / 化工與生化工程研究所 / 101 / In this study, we sought individually the optimum extraction conditions of functional compounds from Chlorella vulgaris C-C by supercritical carbon dioxide fluid (SFE-CO2) with ethanol modifier using two-factors and response surface methodology (RSM).
In two-factors methodology, the optimum conditions of polysaccharides under Chlorella vulgaris C-C weight 1.0 g were extraction pressure 4500.0 psi, extraction temperature 60℃, aqueous ethanol composition 80.0 %, addition volume of aqueous ethanol 70.0 mL, statics extraction time 45.0 min, dynamic extraction time 45.0 min, and CO2-flow rate 8.0 NL/min. Under the optimum conditions, the extract efficiency of 8 cycle batch extraction of polysaccharides was 560.343 mgGlucose/g.
In response surface methodology, the extraction yield of polysaccharides has a deep impact on extraction pressure and extraction temperature by analysis of variance under Chlorella vulgaris C-C weight 1.0 g, statics extraction time 45.0 min and dynamic extraction time 45.0 min. The optimum conditions of polysaccharides under Chlorella vulgaris C-C weight 1.0 g, statics extraction time 45.0 min and dynamic extraction time 45.0 min were extraction pressure 4742.31 psi, extraction temperature 66.55℃, aqueous ethanol composition 80.0 %, addition volume of aqueous ethanol 40.0 mL, and CO2-flow rate 4.0 NL/min. Under the optimum conditions, the extract yield of 8 cycle batch extraction of polysaccharides was 515.63 mgGlucose/g;The extraction yield of polyphenols has a great impact on extraction temperature and aqueous ethanol composition by analysis of variance. The optimum conditions of polyphenols under Chlorella vulgaris C-C weight 1.0 g, statics extraction time 45.0 min, and dynamic extraction time 45.0 min were extraction pressure 4500.0 psi, extraction temperature 68.13℃, aqueous ethanol composition 87.94 %, addition volume of aqueous ethanol 40.0 mL, and CO2-flow rate 4.0 NL/min. Under the optimum conditions, the extract yield of 8 cycle batch extraction of polyphenols was 63.87 mgGallic acid/g; The extraction yield of flavonols has a profound impact on extraction temperature and aqueous ethanol composition by analysis of variance. The optimum conditions of flavonols under Chlorella vulgaris C-C weight 1.0 g, statics extraction time 45.0 min, and dynamic extraction time 45.0 min were extraction pressure 4500.0 psi, extraction temperature 71.14℃, aqueous ethanol composition 93.08 %, addition volume of aqueous ethanol 70.0 mL, and CO2-flow rate 4.0 NL/min. Under the optimum conditions, the extract yield of 8 cycle batch extraction of flavonols was 111.27 mgQuercetin/g; The extraction yield of flavanones has a great impact on extraction temperature and aqueous ethanol composition by analysis of variance. The optimum conditions of flavanones under Chlorella vulgaris C-C weight 1.0 g, statics extraction time 45.0 min, and dynamic extraction time 45.0 min were extraction pressure 4500.0 psi, extraction temperature 49.75℃, aqueous ethanol composition 80.0 %, addition volume of aqueous ethanol 56.3 mL, and CO2-flow rate 4.0 NL/min. Under the optimum conditions, the extract yield of 8 cycle batch extraction of flavanones was 83.11 mgHesperetin/g; The extraction yield of carotenoids has a deep impact on extraction temperature and aqueous ethanol composition by analysis of variance. The optimum conditions of carotenoids under Chlorella vulgaris C-C weight 1.0 g, statics extraction time 45.0 min, and dynamic extraction time 45.0 min were extraction pressure 4500.0 psi, extraction temperature 60.43℃, aqueous ethanol composition 90.65 %, addition volume of aqueous ethanol 70.0 mL, and CO2-flow rate 4.0 NL/min. Under the optimum conditions, the extract yield of 8 cycle batch extraction of carotenoids was 10.11 mg/g; The extraction yield of chlorophyll a has a deep impact on extraction pressure and aqueous ethanol composition by analysis of variance. The optimum conditions of chlorophyll a under Chlorella vulgaris C-C weight 1.0 g, statics extraction time 45.0 min, and dynamic extraction time 45.0 min were extraction pressure 4500.0 psi, extraction temperature 65.73℃, aqueous ethanol composition 91.45 %, addition volume of aqueous ethanol 70.0 mL, and CO2-flow rate 10.0 NL/min. Under the optimum conditions, the extract yield of 8 cycle batch extraction of chlorophyll a was 26.06 mg/g.
The extraction yield obtained from two-factors and RSM, they could be fitted by = N=1 A (1 - exp(-N(tS + tD)/B)) equation. In this study, the extraction yields are high than compared records because SFE-CO2 with ethanol modifier can effectively extract the functional compounds from Chlorella vulgaris C-C cell wall.
| Identifer | oai:union.ndltd.org:TW/101KYIT0566006 |
| Date | January 2013 |
| Creators | Chen Yu Wei, 陳昱瑋 |
| Contributors | Pan Jianliang, 潘建亮 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 183 |
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