碩士 / 銘傳大學 / 生物科技學系碩士班 / 101 / The aim of this study is to isolate and characterize bacteriocin-like inhibitory substance (BLIS)-producing lactic acid bacteria from the intestine of grey mullet. A total of 194 acid-producing bacteria were isolated and four isolated cultures showed inhibitory activities against at least one indicator strain. Strains Enterococcus faecium B3-8 and Ent. thailandicus B3-22 showed the greatest inhibitory activities against Listeria monocytogenes and were therefore further characterized. However, the BLISs from B3-8 and B3-22 showed different characteristics regarding heat sensitivity and antibacterial spectra and were therefore considered as two different inhibitory substances. Besides, it was also found that BLIS from strain B3-22 had a significant inhibitory ability against Lactococcus garvieae strains. The biocontrol ability of strain B3-22 against the Lactococcus garvieae and the potential cell wall-degrading ability of its bacteriocin were therefore further evaluated. The results suggested that the growth of Lactococcus garvieae can be effectively inhibited using either the living culture or the partial purified bacteriocin substance of strain B3-22. The results also indicated that BLIS from strain B3-22 has cell wall-degrading ability against L. garvieae, and can work synergistically with lysozyme. The findings obtained in this study suggest that Ent. thailandicus B3-22 has obvious potential for practical application as a biopreservative in the aquaculture, due to its excellent inhibitory activity against L. garvieae.
| Identifer | oai:union.ndltd.org:TW/101MCU05111003 |
| Date | January 2013 |
| Creators | Yu-Hsuan Lin, 林郁軒 |
| Contributors | Yi-Sheng Chen, Hui-Chung Wu, 陳奕伸, 吳慧中 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 60 |
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