碩士 / 國立中興大學 / 化學工程學系所 / 101 / This study investigated ultrasonic solvent extraction, normal phase column elution fractionation and supercritical anti-solvent (SAS) precipitation of fucoxanthin from brown algae of Hincksia mitchellae P.C. Silva. The 5.5 mg/g content of fucoxanthin in algae was evidenced by soxhlet ethyl acetate extraction at 353 K and 16 hours. The 5.4 mg/g content of fucoxanthin of extract was obtained by ultrasonic ethyl acetate extraction at temperature (298 K), solvent to solid ratio (60:1), time (10 min) and continuous 3 times of extraction. Ultrasonic extract eluted by normal phase column fractionations with a mixed solvent of n-hexane and acetone (7:3), then we can get 719.8 mg/g content of fucoxanthin in fraction with an average recovery of 85.4%. preliminary SAS diethyl ether solution precipitation of the column fractions were carried out by investigating the effect of temperature, pressure, retention time, feed flow rate and feed concentration on purity and recovery of fucoxanthin. Two-factor (pressure and time) schemed response surface methodology designed SAS experiments produced micro-sized irregularly flower-type agglomerated precipitates containing 886.2 mg/g of fucoxanthin with a recovery of 98.7% and showed that pressure is a major factor on enhancing purity of fucoxanthin. The result also showed SAS is prior to liquid-liquid anti-solvent precipitation of the fucoxanthin content of 790.5 mg/g. Dissolution rate tests showed the SAS product was faster dissolved in aqueous solution than that of the liquid-liquid anti-solvent precipitates and brown algae powders.
| Identifer | oai:union.ndltd.org:TW/101NCHU5063090 |
| Date | January 2013 |
| Creators | Da-Ming Lin, 林達明 |
| Contributors | Chieh-Ming Chang, 張傑明 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 63 |
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