碩士 / 國立中興大學 / 生命科學系所 / 101 / To solve the energy shortage problem, the alternate energy methane (gas) hydrates which are estimated at about twice the amount of all other known fossil fuel reserves, are distributed along coastal margins, like SW offshore of Taiwan, as well as in terrestrial regions and marine sediments. With 74% of methane on earth are biogenic origin, this represents a significant source of natural gas from biological methanogenesis. Isotopic data from professor Yang Tsan-Yao at National Taiwan University suggested that most of the methane produced in Taiwan methane hydrate potential area is biogenic origin. To explore the methanogen diversity at methane seep or hydrate habitats, sediment samples obtained from piston and gravity cores at 96 Mud Volcano groups, Fangliao Canyon and KP-9 region by ORI and ORIII cruises were anaerobic enriched for methanogens. After serial sub-transfer and anaerobic roll-tube incubation, genus as Methanoculleus, Methanofollis, Methanosarcina, Methanococcoides and Methanogenium were partially purified. Among them, the irregular coccoid cells of Methanoculleus sp. S3Fa from the core sediment of 96 Mud Volcano Groups were purified. The 16S rDNA sequence of the strain S3Fa showed 99 % similarity with Methanoculleus marisnigri and M. submarinus (isolated from methane hydrate at Nankai Trough, Japan). Comparison of the 16S rRNA sequences of sediment samples with related uncultured environment samples showed that methanogenic phylotypes are close related with clones from marine sediment of submarine mud volcanos at East Mediterranean Sea, methane hydrate-bearing sites and oil reservoir sites. The results suggests that those phylotype of methanogens maybe important in methane hydrate formation.
| Identifer | oai:union.ndltd.org:TW/101NCHU5105122 |
| Date | January 2013 |
| Creators | Mei-Fei Chen, 陳眉霏 |
| Contributors | 賴美津 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 92 |
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