碩士 / 國立中興大學 / 獸醫學系暨研究所 / 101 / Shewanella is a facultatively anaerobic gram-negative bacillus. It is a saprophytic, marine organism which is often associated with opportunistic infections. Most reported cases had history of sea water exposure or seafood consumption. Recently, case reports of Shewanella infection have increased. Many of them were found in the Asian region and Taiwan. The study aims to investigate the occurrence, distribution, and diversity of Shewanella spp. in Taiwan coastal water and aquaculture. In total, 80 aquaculture samples and 25 water samples were collected. Shewanella was identified to species level using 16S-rRNA sequences. More characteristics were analyzed using auxiliary biochemical, growth, and hemolytic tests. A total of 64 Shewanella strains were isolated from aquaculture samples, included S. haliotis, S. algae, S. chilikensis, S. marisflavi, S. aquimarina and S. loihica. 22 Shewanella strains were isolated from water samples, included S. haliotis, S. algae, S. marisflavi, S. aquimarina, S. loihica, S. putrefaciens and S. baltica. The majority of Shewanella species amongst the Shewanella isolates were S. algae, S. haliotis and S. putrefaciens. Most Shewanella isolates are able to grow at 37℃, in the presence of 6.5% NaCl and exhibit hemolysis on sheep blood agar. Further studies are required to identify its virulence factors and role in diseases. Our results suggest that aquaculture are reservoirs of opportunistic pathogenic microorganisms to aquatic organisms and humans and a better understanding of the microbial ecology of the aquaculture will provide insights to the possible health hazards from these microorganisms.
| Identifer | oai:union.ndltd.org:TW/101NCHU5541035 |
| Date | January 2013 |
| Creators | Shu-Ying Tseng, 曾淑瀛 |
| Contributors | 董光中 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 68 |
Page generated in 0.0071 seconds