碩士 / 國立中興大學 / 獸醫學系暨研究所 / 101 / The aim of this study is to develop of the formalin detection technique by High-performance liquid chromatography (HPLC) and determine the residual concentration of formalin in muscle and liver of sturgeon and rainbow trout. Our results showed, our developed HPLC method set with C18 coloume, acetonitrile: DDW (20 : 80) as a mobile phase (pH 7) , flow rate 1 mL/min, and the UV-detector detects the max peak at wavelength 360 nm. Our results suggested that the median lethal concentration (LC50) lowered progressively as the duration of exposure increased. The toxicity curves became almost asymptotic after treatment 96 h. The 96h-LC50 of sturgeon and rainbow trout was 182.4 and 179 ppm at 19℃, respectively. After 50 ppm formalin treatment, fishes has been found to cause gill lamellar epithelia separation, hypertrophy, and necrosis under the histopathology examination. However, the gills had recovered after 96 h. The time of undetectable residue amount of formalin in the liver and muscle tissue of the sturgeon at 25 and 50 ppm was (48 hr and 96 hr) and (0 hr and 0 hr) , respectively. At the same way, the time of undetectable residue amount of formalin in the liver and muscle tissue of the rainbow trout at 25 and 50 ppm was (96 hr and 240 hr) and (8 hr and 24 hr) , respectively. Thus, to use low concentration of formalin in the edible tissues of cold water fish is safe and recommend. We recommended withdrawal period for sturgeon and rainbow trout were 8 and 20 days.
| Identifer | oai:union.ndltd.org:TW/101NCHU5541036 |
| Date | January 2013 |
| Creators | Hsin-Yao Huang, 黃忻曜 |
| Contributors | Way-Shyan Wang, 王渭賢 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 99 |
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