碩士 / 國立成功大學 / 生物科技研究所碩博士班 / 101 / Innate immune system is important for the disease resistance of fish. The innate immunity of fish is divided in three parts: physical barriers, humoral and cellular components, which are common to resistance variety water-pathogen infection and maintain the healthy statement of fish. Interleukin-6 (IL-6) is a cytokine of the lymphocytes hormone (lymphokine), mainly induce lymphocytes to reaction. It plays important roles in the innate and acquired immunity. In the research of mammalian, IL-6 can induce acute phase proteins (APPs) and antimicrobial peptide genes expression as well as stimulated the macrophage and neutrophil activation and regulate the inflammatory cytokines expression in the innate immunity, then enhance the resistance to bacterial disease. Regarding to the acquired immunity, IL-6 promotes T cell and B cell differentiation and increases the antibody production. In teleost fish, the functions of IL-6 are not confirmed yet. Previous studies in our laboratory use Epinephelus coioides to be the animal model, confirmed that IL-6 induce the Th2 pathway in the acquired immunity and enhance antibody production. In my study, investigate the mechanism of IL-6 in innate immunity and evaluate its efficacy of disease resistance. In the current study, inject of recombinant IL-6 (rIL-6) regulate some inflammatory cytokine, antimicrobial peptide and other innate immunity related genes. Further, we comfirm the antibacterial activity in serum by rIL-6 induction, can reduced the bacterial number in the in vitro test, and enhance the phagocytosis of head kidney leukocytes. Finally, in the in vitro challenge test, fish injection of rIL-6 was highersurviver while charllenged by Vibrio campbellii and Nervous Necrosis Virus, and reduced the pathogen. In summary, grouper IL-6 was a molecular that could induce multiple innate immune reactions, which will help to resistant the pathogen invasion.
| Identifer | oai:union.ndltd.org:TW/101NCKU5111121 |
| Date | January 2013 |
| Creators | Yi-FanFang, 方苡帆 |
| Contributors | Han-You Lin, 林翰佑 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 102 |
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