碩士 / 國立成功大學 / 生物科技研究所碩博士班 / 101 / Grouper aquaculture industry is considered as one of a potential economic industries in Taiwan, and orange-spotted grouper (Epinephelus coioides) is the main breeding species. However, in recent years, nervous necrosis virus (NNV) leads to high mortality rate, and cause serious crop losses of fishermen. According to previous studies in our laboratory, virus infection will turn on immune gene to protect the fish, which mostly induced by interferon (IFN). IFN was induced by virus infection to open the downstream antiviral mechanism, therefore, this study will invesgate the IFN expression in different post infection time and organs, to realize the activation of antiviral protein. In here, we cloned the sequence first. OsgIFN contain 769 base pairs, encoding 172 amino acid and one signal peptide. Classfication of osgIFN belongs to 2C-IFN. According to the real-time PCR results, osgIFN mRNA level increase with the infection time, and the significantly increasing trend was observed in immune organ like head kidney and intestine. On the other hand, IFN was know to be screated cytokine, the gene will turn on after virus infection, and then the IFN protein secreated from cell to cell, leads the neighboring cells induce antiviral protein. Therefore, at the part of antiviral activity investigation, osgIFN recombinant protein treatment will promote Mx promoter activity in GF-1, shows that osgIFN can actually induced Mx gene. At the part of tissue distribution analysis of Mx gene induced by osgIFN, the results from real-time PCR show that there are significantly increasing trend in head kidney and eye, indicating that the susceptibility is higher in the immune organ and nervous system.
| Identifer | oai:union.ndltd.org:TW/101NCKU5111129 |
| Date | January 2013 |
| Creators | Guan-RuChen, 陳冠儒 |
| Contributors | Tzong-Yueh Chen, 陳宗嶽 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 104 |
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