碩士 / 國立成功大學 / 環境工程學系碩博士班 / 101 / Climate change is a growing concern. Most of the researches indicate that it is mainly resulted from green house gases (GHGs) emission among which carbon dioxide plays the major source. To mitigate climate change, carbon capture and storge (CCS) has been applied worldwide to capture and store carbon dioxide.
Biofixation using microalgae has recently become an attractive approach to CO2 capture and recycling with additional benefits of downstream utilization and applications of the produced microalgal biomass. Microalgae produces nutraceutical product C-phycocyanin (C-PC) and simultaneously mitigates CO2 emissions during its growth. Phycocyanin (PC) is a blue, light-harvesting pigment in cyanobacteria. C-PC is considered as a healthy ingredient in cyanobacterial-based foods and health foods with fluorescent and antioxidative properties. It can be used as nutrient ingredients and natural dyes for food and cosmetics potential therapeutic agent in oxidative stress-induced diseases, and as fluorescent markers in biomedical research.
Light intensity and light quality are two critical factors that influence the accumulation of phycobiliproteins in microalgae. In addition, as outdoor cultivation in the future is considered, the research of the light/dark cycle which affects the production of C-PC is also need. In this study, a thermophilic cyanobacterium named Thermosynechococcus sp. CL-1 (TCL-1) was cultivated to evaluate the effects of light intensities(500、1,000、1,500 and 2,000 μE m-2 s-1), light quality (white light and red light) and light/dark cycle(light: dark=12h:12h) on the CO2 fixation ability and C-PC production.
The result indicates that there was the maximum biomass productivity 1.35 g L-1 d-1 in continuous cultivation at light intensity of 2,000 μE m-2 s-1(red light). The maximum CO2 fixation rate was 2.29 g L-1 d-1 in continuous cultivation at light intensity of 2,000 μE m-2 s-1(white light). The maximum C-PC productivity was 123 mg L-1 d-1 in continuous cultivation at light intensity of 1,000 μE m-2 s-1(white light).
According to the results of this study, the greatest influence factor of the CO2 fixation rate and C-PC productivity was the biomass productivity, followed by the carbon and C-PC content of the cell. After comparing all the factors in this study, it was found that there was an economic advantage for TCL-1 cultivated in continuous illumination at light intensity of 1,000 μE m-2 s-1(white light) as its biomass productivity, CO2 fixation rate and C-PC are considered.
| Identifer | oai:union.ndltd.org:TW/101NCKU5515027 |
| Date | January 2013 |
| Creators | Yu-TingLin, 林鈺婷 |
| Contributors | Hsin Chu, 朱信 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 160 |
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