碩士 / 國立彰化師範大學 / 電機工程學系 / 101 / We demonstrate the use of a fiber-optic spectrometer for real time in vivo monitoring the liver of transgenic zebrafish. Excessive production of reactive oxygen species (ROS) is implicated in chronic hepatitis and liver cancer. Superoxide anion (O2−) is the major ROS generated in mitochondria. Recently, a circularly permuted yellow fluorescent protein (cpYFP) is developed as a superoxide-specific indicator. Because the genes of zebrafish are similar to those of vertebrates and the skin and organs of zebrafish juvenile are transparent, which make cpYFP-transfected zebrafish a suitable animal model for the study of superoxide production in liver. We first measured different concentrations (1.0×10-3 M~1.0×10-6 M) of fluorescein isothiocyanate (FITC) to prove the quantification ability of the fiber-optic spectrometer. We then measured the spectra of different fluorescent proteins in transgenic zebrafishes to demonstrate the capability of quantification of multiple fluorescent proteins in vivo. Finally, we treated cpYFP-transfected zebrafish with superoxide-generating agents (paraquat, thioacetamide, and Cu2+) and superoxide-scavenging agents (vitamin C and silymarin) to investigate the dynamics of superoxide anions in the liver in vivo in real time.
| Identifer | oai:union.ndltd.org:TW/101NCUE5442040 |
| Date | January 2013 |
| Creators | 許哲偉 |
| Contributors | 張譽鐘 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 69 |
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